NERDG 2026
Poster 22 Abstract
Comparative Evaluation Of Spray Drying And Freeze Drying Using Different Stabilizers For Solidification Of Nanosuspension
Shashank Reddy Pasika, Vishvesh Raje, Yi Guo
College of Pharmacy and Health Sciences, St. John’s University, Queens, New York-11439 USA
Presenting Author: Shashank Reddy Pasika
Corresponding Author: Ketan Patel, [email protected]
Purpose
Nanosuspension is an approach for enhancing the dissolution of poorly soluble drugs by reducing the particle size and increasing the surface area. However, they are thermodynamically unstable in liquid form, leading to aggregation and crystal growth. Currently, freeze-drying and spray drying are strategies used for solidifying nanosuspensions to improve their physical stability. The study aims to investigate the impact of various excipients, including mannitol, L-leucine, trehalose, lactose, and β-cyclodextrin, on the stabilization of nanosuspensions after solidification. The nanosuspensions are formulated using poorly soluble BCS class II model drugs, Fenofibrate and Albendazole. Evaluating parameters, such as particle size and PDI, of the redispersed solid powder formulated using two technologies, spray drying and freeze drying.
Methods
Fenofibrate and albendazole nanosuspensions were prepared using dual centrifugation wet media milling with 0.8 mm beads at a milling speed of 1000 rpm for 90 minutes at a milling temperature of -20 °C. The nanosuspensions were prepared using a previously optimized method with 2.5% HPMC E5 and 0.2% SLS, varying the drug loading. The prepared nanosuspension was further spray-dried (Buchi Mini B-90 spray dryer) with 2% solid content along with the addition of 2.5% bulking agents. The process parameters include inlet temperature: 95-105°C, pump rate: 15-20%, and outlet temperature: 50°C. The bulking agents used are mannitol and L-Leucine. The same liquid nanosuspension was freeze-dried, and the results were compared using particle size, PDI, and drug content.
Results
The formulated FNF and ABZ nanosuspensions with 2 and 4% drug showed particle size (<260 nm) and PDI (<0.2). The spray-dried and freeze-dried formulations showed an increase in particle size of ≥20nm using mannitol as a stabilizer. Both 2% and 4% drug loading did not show a significant difference in particle size and PDI using mannitol. However, the spray-dried formulation using L-leucine increased the particle size by ≥150nm for both ABZ and FNF. The study focuses on utilizing various pharmaceutical excipients, including trehalose, sucrose, β-cyclodextrin, and lactose.
Keywords
Nanosuspension, spray drying, freeze drying, stabilization, solid nanosuspension
Poster 22 Abstract
Comparative Evaluation Of Spray Drying And Freeze Drying Using Different Stabilizers For Solidification Of Nanosuspension
Shashank Reddy Pasika, Vishvesh Raje, Yi Guo
College of Pharmacy and Health Sciences, St. John’s University, Queens, New York-11439 USA
Presenting Author: Shashank Reddy Pasika
Corresponding Author: Ketan Patel, [email protected]
Purpose
Nanosuspension is an approach for enhancing the dissolution of poorly soluble drugs by reducing the particle size and increasing the surface area. However, they are thermodynamically unstable in liquid form, leading to aggregation and crystal growth. Currently, freeze-drying and spray drying are strategies used for solidifying nanosuspensions to improve their physical stability. The study aims to investigate the impact of various excipients, including mannitol, L-leucine, trehalose, lactose, and β-cyclodextrin, on the stabilization of nanosuspensions after solidification. The nanosuspensions are formulated using poorly soluble BCS class II model drugs, Fenofibrate and Albendazole. Evaluating parameters, such as particle size and PDI, of the redispersed solid powder formulated using two technologies, spray drying and freeze drying.
Methods
Fenofibrate and albendazole nanosuspensions were prepared using dual centrifugation wet media milling with 0.8 mm beads at a milling speed of 1000 rpm for 90 minutes at a milling temperature of -20 °C. The nanosuspensions were prepared using a previously optimized method with 2.5% HPMC E5 and 0.2% SLS, varying the drug loading. The prepared nanosuspension was further spray-dried (Buchi Mini B-90 spray dryer) with 2% solid content along with the addition of 2.5% bulking agents. The process parameters include inlet temperature: 95-105°C, pump rate: 15-20%, and outlet temperature: 50°C. The bulking agents used are mannitol and L-Leucine. The same liquid nanosuspension was freeze-dried, and the results were compared using particle size, PDI, and drug content.
Results
The formulated FNF and ABZ nanosuspensions with 2 and 4% drug showed particle size (<260 nm) and PDI (<0.2). The spray-dried and freeze-dried formulations showed an increase in particle size of ≥20nm using mannitol as a stabilizer. Both 2% and 4% drug loading did not show a significant difference in particle size and PDI using mannitol. However, the spray-dried formulation using L-leucine increased the particle size by ≥150nm for both ABZ and FNF. The study focuses on utilizing various pharmaceutical excipients, including trehalose, sucrose, β-cyclodextrin, and lactose.
Keywords
Nanosuspension, spray drying, freeze drying, stabilization, solid nanosuspension
